Cell cycle and apoptosis analysis reveal that the effect of FLT3 mutations alone or in combination with MLL-AF4 on hESC hematopoietic specification is independent of proliferation or survival. (A) Top, representative flow cytometric analysis showing how cell cycle analysis was analyzed in hemogenic precursors and hematopoietic cells. Bottom, similar proportion of cycling (S+G2+M) hemogenic precursors (left) and hematopoietic cells (right) among the distinct transgenic hESCs. (B) Top, representative flow cytometric analysis showing how apoptosis was analyzed in hemogenic precursors and hematopoietic cells. Bottom, similar proportion of apoptotic (Annexin V+) hemogenic precursors (left) and hematopoietic cells (right) among the distinct transgenic hESCs. N.D: not determined because of the complete absence of CD45⁺ cells for analysis.