STAT3 expression analysis. (A-B) STAT3 mRNA expression average (± SE) obtained by RT-PCR and normalized on GAPDH. Panel A reports results from patients’ LGLs (n = 27) compared with control participants’ CTL cells (n = 18). Panel B represents patients’ LGLs compared with autologous PBMCs depleted of LGLs (n = 10). The average differences were statistically significant (P = .0028 and P < .05, respectively). (C) Evaluation of P-STAT3 and total STAT3 by western blot analysis. β-Actin served as a loading control. Representative cases for patients’ LGLs, control participants’ CTLs, and autologous LGL-depleted PBMCs (patients #4 and #5) are reported.