SOCS3 expression analysis. (A-D) SOCS3 expression average (± SE) obtained by RT-PCR and normalized on GAPDH and western blot analysis of cell extracts for SOCS3 in 3 representative patients (LGLs) and 3 control participants (CTLs). β-Actin served as a loading control. Panel A and B report results from patients’ LGLs (n = 27) compared with control participants’ CTLs (n = 18). Panel C and D report data from patients’ LGLs compared with autologous LGL-depleted PBMCs. The differences were statistically significant (panel A, P = .003 and panel C, P = .0015). (E) Mean results (± SE) of SOCS3 mRNA expression and (F) protein level in 2 representative patients and 1 control participant obtained by cells after culture in medium alone or medium with IL-6 for 1 hour.