Figure 2
RAF1S259A induces expression of the entire embryonic arteriogenic program. (A) qPCR of indicated genes in HUVECs infected with empty control, WT HA-RAF1 or HA-RAF1S259A (S259A) lentiviruses. Mean ± SEM. n = 3. (B) Immunoblot of control, RAF1 WT, and S259A lentivirus-infected HUVECs. (C) Immunoblot of RAF1 WT and S259A adenoviruses-infected HUVECs. (D) qPCR of E10.5 yolk sacs. Control, n = 10 embryos; S259A, n = 6 embryos. Mean ± SEM. HA, hemagglutinin; mRNA, messenger RNA; NS, not significant.
