TDZD-8 specifically induces cell death of primary leukemia specimens. (A) Primary AML (n = 37), CLL (n = 12), ALL (n = 6), bcCML (n = 6), and normal mononuclear cells (n = 13, obtained from BM [n = 3], CB [n = 7], or MPB [n = 3]) were cultured for 18 to 24 hours in the presence of 20 μM TDZD-8. Cell viability was assessed by Annexin V/7-AAD staining. Percent viability is represented relative to untreated control. Leukemia specimens were significantly (P < .001) more sensitive to TDZD-8 than were normal specimens. Error bars represent the SEM. All assays were performed in triplicate. (B) Representative dot plots for the 24-hour flow cytometric analysis with Annexin V/7-AAD stain in the presence or the absence of 20 μM TDZD-8.