The effect of PI3-K inhibitors on Akt phosphorylation induced by IGF-1 in platelets. (A) Washed platelets preincubated with 25 μM LY294002, 100 nM wortmannin, 500 nM PIK-75, 500 nM TGX-221, 2 μM AS-252424, or 1 μM IC87114 were stimulated at 37°C for 3 minutes with either 1 μM IGF-1. (B) Platelets preincubated with 100 nM wortmannin, 500 nM PIK-75, 500 nM TGX-221, or 2 μM AS-252424 were stimulated at 37°C for 3 minutes with either 100 nM 2-MeSADP, 100 nM IGF-1, or a combination of 100 nM 2-MeSADP and 100 nM IGF-1, as noted. The reaction was stopped by the addition of 3 × SDS sample buffer. Equal amounts of proteins were analyzed by Western blot analysis with anti-Ser(P)⁴⁷³ or anti-PKCδ (lane loading control) antibody. The Western analysis shown is a representative of 3 independent experiments.