IL-6 stimulation induces colocalization of HGAL with actin and WASP proteins in podosomes. Unstimulated or IL-6–stimulated Hela-HGAL (A) and SUDHL6 (B) cells were fixed with 4% paraformaldehyde and stained with anti-HGAL antibody (green), anti-WASP antibody (green or red, as indicated), rhodamine-labeled phalloidin (red), and DAPI (blue), and were visualized on a Carl Zeiss LSM510 microscope. Arrows indicate podosome-like structures. Representative photographs of 3 independent experiments are shown (see “Immunofluorescence microscopy” for image acquisition information).