HGAL mediates the inhibitory effects of IL-6 on cell migration. (A) CD77⁺ GC B cells and CD77⁻ cells were enriched from normal tonsils as described in “RNA isolation and reverse transcription reaction.” RNA was extracted and HGAL RNA expression was measured by real-time reverse transcription–PCR in triplicates as described in “Real-time PCR measurement of HGAL mRNA expression.” (B) Enriched CD77⁺ GC B cells and CD77⁻ tonsilar cells were evaluated in triplicate in IL-6 chemotaxis assay, as described in “Materials and methods.” Means and standard deviations of 2 independent experiments are demonstrated. (C) VAL lymphoma cells were transfected with control or HGAL siRNA. At 48 hours after siRNA transfection, the cells were used for IL-6 chemotaxis assay performed in triplicate, as described in “Chemotaxis and wound assays.” Means and standard deviations of 3 independent experiments are demonstrated. (D) Western blot analysis of HGAL protein in siRNA-transfected VAL lymphoma cells used for chemotaxis assays shown in panel C.