Effect of ING4 suppression in HMCLs on the production of the proangiogenic molecules. JJN3 and RPMI-8226 were transfected by electroporation with 1 nmol smart pool double-stranded RNA oligonucleotides (siRNA) against ING4 or with a nonspecific control siRNA (Cy). After 24 hours, cells were harvested for ING4 evaluation by RT-PCR (A). The expression of the proangiogenic molecules IL-8, VEGF, ANG-1, and OPN was evaluated by RT-PCR in both JJN3- and RPMI-8226–transfected cells (B). After 48 hours, IL-8 (JJN3 and RPMI-8226) and OPN (RPMI-8226) levels were measured in the conditioned media of transfected cells by ELISA. Graphs represent the mean plus or minus SD of IL-8 and OPN levels normalized for the number of cells (C).