Fractalkine has the greatest effect on chemoattraction. Transwell migration assay results after 3 hours, showing total cell numbers of PBMC populations migrating in response to chemokines present in coculture supernatants from the CMV antigen-stimulated CD4⁺ T cells with endothelial monolayer samples. Sample sets on x-axis are as follows. Sample set 1 was total cell numbers of PBMC populations transmigrating in response to untreated coculture supernatants; sample set 2 was PBMCs treated with anti–human CX₃CR1 antibody transmigrating in response to coculture supernatants; sample set 3 was PBMCs transmigrating in response to coculture supernatants treated with anti–human RANTES antibody; sample set 4 was PBMCs treated with anti–human CX₃CR1 antibody and anti–human RANTES antibody; and sample set 5 was PBMCs treated with isotype antibodies rabbit IgG and mouse IgG. Each sample represents the results from 3 replicate transwell migrations in which the transmigrated populations were counted from each well independently. Results are shown as the mean (± SD) and are representative of 5 assays with different seropositive donors.