Migration of different PBMC cell types in response to chemokine gradients in coculture supernatants. Results are shown as mean (± SD) of transmigrated populations from cell migration assays of 3 CMV-seropositive donors. PBMCs from each donor were prepared for transwell migration assays, as described in “Transwell migration assays and specific antibody neutralization assays.” PBMCs were loaded into the upper chambers of transwells to test migration into coculture supernatants prepared previously from the same donor, or negative control media. The “+” and “−” symbols refer to migration results using coculture supernatants or negative control media, respectively. Culture supernatants from fractalkine-induced cultures were used for the endothelial damage assays. Percentage values with each cell type represent the mean number of cells migrating of each cell type (ie, mean cells migrated in lower chamber/mean cell initially in upper chamber × 100).