CX3CR1 block has greatest inhibition effect on migration of CD14+ cells compared with other PBMC subsets. Untreated PBMCs, CX3CR1 blocked, or RANTES blocked cells or supernatants were compared for migration into antigen-stimulated coculture supernatants. Migrated cells were then collected, stained, and analyzed by flow cytometry to identify migrated subsets of CD14+, NK (CD16+/CD56+), CD4+ T cells, and CD8+ T cells. Treated samples for each analysis are grouped together for PBMCs or each subset as no antibody treatment, anti–CX3CR1-neutralizing antibody treatment, and anti–RANTES-neutralizing antibody. Results are shown as mean (± SD) percentage of migrated cell numbers relative to untreated PBMC migrations for PBMCs, CD14+ cells, NK cells, CD4+ T cells, and CD8+ T cells.
