Decreased in vivo migration of WAS KO DCs compared with C57BL/6 DCs. DCs were CFSE labeled in vitro and injected subcutaneously into wild-type C57BL/6 recipients. DC migration was assessed by quantifying the number of CFSE⁺ DCs in the draining lymph nodes. (A) WAS KO DCs showed a decreased ability to migrate to the draining lymph nodes. (B) Quantification showed that both in unprimed and TNF-α–primed mice, WAS KO DCs have decreased migration in vivo. (C) Injection of a 1:1 mixture of C57BL/6 CFSE⁺ DCs and WAS KO DiI⁺ DCs confirmed that migration of WAS KO DCs was reduced compared with C57BL/6 DCs. (D) A similar increase in lymph node cellularity induced by migration was observed for both WAS KO and C57BL/6 DCs. Fluorescence-activated cell sorting (FACS) plots shown in panel A are after 24 hours and representative of 6 mice. Data shown in panels B and D are averages plus or minus SEM of 2 to 6 mice per group, *P < .05. Data shown in panel C are representative of 2 mice.