Decreased in vivo migration and localization of WAS KO DCs. (A) Immunofluorescent analysis of draining lymph nodes after in vivo migration showed that the WAS KO DCs that reached the lymph nodes did not penetrate into the T-cell area as deeply as C57BL/6 DCs. (B) Quantification of DC localization showed that C57BL/6 DCs penetrated deeper into the T-cell area, compared with WAS KO DCs. Pictures in panel A are representative of 4 mice and of at least 2 independent experiments. CFSE⁺ DCs are green; T cells, red; and B cells, blue. Arrows point to cells that have migrated into the T-cell area. Scale bars are 75 mm. Data shown in panel B are averages plus or minus SEM of 2 to 4 mice per group.