Decreased in vivo migration of WAS KO DCs induces reduced OT-1 proliferation. CD8⁺ OT-I T-cell proliferation was determined after injection of WAS KO or C57BL/6 DCs. In both the draining lymph node (A) and in spleen (B), a reduced proliferation of OT-I cells was observed when WAS KO was injected. Quantification of the data shows that in panel C lymph node a strong reduction is observed, while in panel D spleen the effect is much smaller. Data shown are representative of 3 mice in panels A,B, and averages plus or minus SD in panels C,D.