Effect of KL with and without Dex on HbF content, HbF synthesis, and MCH in β-TI and β-TM. β-TI (left panels) and β-TM (right panels) HPCs were grown in unilineage erythroid minibulk cultures in absence (C) or presence of KL (100 ng/mL), alone or in combination with Dex (10−8 M). Mature erythroblasts were analyzed for their HbF content (top panels), HbF synthetic potential (middle panels), and total Hb content (MCH, bottom panels). With respect to HbF content, mean (± SEM) values from 10 (10 donors) and 7 (7 donors) separate experiments on β-TI and β-TM, respectively, are presented. For HbF synthesis, single experiments (4 and 7 for β-TI and β-TM, respectively) and means (horizontal bar) are shown. Bottom: mean values (± SEM) of MCH levels from 6 separate experiments on β-TI and β-TM, respectively are presented. Variance analysis was expressed as Bonferroni P values. *P < .05, **P < .01, ***P < .001.
