Six-species alignment of the central 235 bp of the + 23 Runx1 enhancer. A stretch of 127 bp is conserved down to frog with 64% sequence identity. Within this stretch, phylogenetic footprints without (A-C) and with exact matches to predicted consensus TF-binding sites are indicated. In addition, mouse-chicken conserved Ets motifs at the 5′ and 3′ end of the enhancer fragment, and a mouse-opossum conserved E-box, are indicated. Mm indicates Mus musculus; Hs, Homo sapiens; Cf, Canis familiaris; Md, Monodelphis domestica; Gg, Gallus gallus; and Xt, Xenopus tropicalis.