In vivo TF binding at the + 23 Runx1 enhancer. (A) Schematic representation of the relative positions of 5 Taqman probes in Runx1 intron 1 and surrounding the + 23 enhancer. Overlap of probes with CNEs as indicated. M indicates mouse; D, dog; and F, frog. (B) Real-time PCR analysis of ChIP with antibodies directed against Gata2, Runx1, Fli-1, Elf-1, Pu.1, Scl, Lmo2, and Ldb1 in 416B cells. A no-antibody (no Ab) control was included in each ChIP experiment. Data are the mean (± SD) from 2 (Gata2, Runx1, Fli-1, Elf-1, Pu.1, Scl) or one (Lmo2, Ldb1) independent ChIP with 3 real-time PCR assays per ChIP.