Lytic activity of CTLs generated from patient 643. Autologous iDCs were transfected with either CsiRNA (□/○) or iPsiRNA (■/●). After the induction of DC maturation, these DCs were transfected with either MAGE-3 RNA (A) or tyrosinase RNA (B) and then used to stimulate autologous T cells in culture. After 1 restimulation, lytic activity was determined in duplicate against autologous DCs transfected with RNA encoding either MAGE-3 or tyrosinase, and against the autologous melanoma cell line 643 left untreated or preincubated with IFN-γ for 3 days in culture. Results are plotted as mean values (± SD); *P < .05.