Analysis of mFox-2 isoform expression and its effect on exon 16 splicing switch during MELC differentiation. (A) Schematic diagram of primer sets used in expression analysis. Primer set locations are indicated with arrows. (B) Real-time PCR analysis of total mFox-2 (mFox-2), mFox-2F, and mFox-2A expression during MELC differentiation. 0, 2, 4, and 6 indicate the days after DMSO-induced differentiation. All data are presented as mean plus or minus SD. Day 0 was taken as 1. (C) Full-length Fox-2 mRNA expression detected during MELC differentiation. mFox-2 indicates total mFox-2; Hprt1 serves as an internal control. (D) mFox-2 isoform expression switch correlates with exon 16 splicing switch during MELC erythroid differentiation. 0, 2, 4, and 6 indicate the days of induced differentiation. Top panel shows endogenous mFox-2 isoforms expression was detected by anti–mFox-2 antibody. Middle panel shows β-actin was used for loading control. Bottom panel shows exon 16 splicing was analyzed by semiquantitative RT-PCR from 3 independent transfections.