Quantification of CDX2 expression in patients with AML by RQ-PCR. (A) Expression levels of CDX2 in patients with AML with a normal karyotype with (NPMc⁺) or without NPM1 mutation (NPMc⁻) or (B) in different AML subgroups with abnormal karyotype. A total of 115 samples were analyzed. The number of patients who were positive for CDX2 expression and were therefore evaluated for expression levels are indicated. ΔC_T values were obtained by normalization for the housekeeping gene TBP; the mean values (± SD) are shown. The expression level of the gene is inversely correlated with the ρC_T value.