Enhanced translocation of AktPH-GFP to the phagocytic cup of Ship1^−/− macrophages in response to serum-opsonized zymosan. Peritoneal macrophages from wild-type (WT; Ship1^+/+) AktPH-GFP Tg, Ship1^−/−AktPH-GFP Tg, or WT GFP Tg mice that had been treated with (■) or without (□) wortmannin were incubated with IgG-coated beads or SOZ. The fluorescence intensity of AktPH-GFP or GFP at the phagocytic cup was normalized to the cytosolic fluorescence intensity. (A) Results are the peak values of the relative intensity in a cell (± SEM or range) from 2 to 5 independent experiments. (B) Time course analyses of the relative fluorescence intensity (phagocytic cup/cytosol) during phagocytosis of SOZ. The time of closure arbitrarily defines the 0 time point. • indicates 100 nM wortmannin treatment, and ○ represents no inhibitor treatment. Data shown are representative of 5 independent experiments. The time course from which these data were obtained is shown in Videos S8 and S9.