Figure 4
TCF-1 binding to β- and γ-catenin. (A) The NH2-terminal domain of TCF-1 (amino acids 1-117) was fused to GST and used to test binding to β- and γ-catenin present in HEK 293T or thymocyte cellular lysates. Lysates were pre-cleared with GST alone, and binding proteins were revealed by immunoblot. (B) Wnt reporter gene activity in response to expression of TCF-1 in combination with β-catenin or γ-catenin as analyzed in 293T cells. Error bars are SD.
