Ectopic expression of PKB regulates eosinophil differentiation ex vivo. CD34⁺ cells were retrovirally transduced with myrPKB, PKBcaax, or eGFP alone. (A) Retrovirally transduced CD34⁺ cells were cultured in presence of IL-5 to induce eosinophil differentiation. After 17 days of culture, transduced cells were separated from the nontransduced cells by FACS, and cytospins were made. Cytospins were stained with May-Grunwald Giemsa solution. (B) Data were expressed as the percentage of differentiated eosinophils. (C) Data were expressed as the percentage of differentiated neutrophils that developed during the 17-day culture period in presence of IL-3 and IL-5. (D) Lactoferrin, (E) CD49d, and (F) CD14 expression was analyzed by FACS to determine neutrophil, eosinophil/monocyte, and monocyte, respectively in presence of IL-3 and IL-5. Error bars represent SEM.