CR3 (CD11b/CD18) mobility increases after CD44 stimulation. (A,B) Confocal fluorescent and transmitted light images of RAW macrophages stained with Alexa Fluor 488–conjugated anti-CD11b prior to CD44-mediated phagocytosis of Ebabs. Arrowheads represent the phagocytic cup. Scale bar represents 5 μm. (C-F) Graphic representation of fluorescence recovery after photobleaching (FRAP) of CD11b Alexa Fluor 488 after photobleaching. Cells were untreated (CTRL) or stimulated with antibody (CTRL isotype and CD44) or with 100 nM PMA. Data are representative of 3 independent experiments. (G-R) Fluorescent images of RAW macrophages stained with Alexa Fluor 488–conjugated anti-CD11b before and immediately after photobleaching and then 5 minutes after photobleaching. Scale bar represents 5 μm. The lines labeled 1 or 2 in panels C-F represent the fluorescence intensity over time of the circles in the corresponding fluorescent images (G-R); 1 being the bleached cell and 2 being the control cell.