DMAPT induces death of primary human AML, ALL, and CML cells, but not normal hematopoietic cells. (A) Percentage of viable cells of primary human AML cells exposed to either 7.5 μM PTL or DMAPT. Viability was measured by labeling with annexin-V and 7-AAD. Analysis of total AML versus selected CD34⁺ cells are indicated. (B) Percentage of viable cells of CD34⁺CD38⁻ AML cells at the indicated concentrations of DMAPT. (C) Percentage of viable cells of CD34⁺CD10⁻ ALL cells at the indicated concentrations of DMAPT. (D) Percentage of viable cells of CD34⁺CD38− CML cells at the indicated concentrations of DMAPT. (E) Percentage of viable cells of normal CD34⁺ or CD34⁺CD38⁻ hematopoietic cells obtained from healthy donors (CB or BM) and treated at the indicated concentrations of DMAPT. In all panels, the horizontal bars represent the mean, and each circle or diamond represents 1 specimen. Analysis of each specimen was performed in triplicate, and the average was used to represent the results for a single specimen. All viability values are relative to untreated controls.