DMAPT demonstrated biological activity in vivo. (A) Overlays for CD34 expression in BM (top) and PB (bottom) from pretreatment (filled histograms) and posttreatment (open histograms) specimens from canine case no. 1. (B) Percentage of canine cell engraftment achieved in NOD/SCID mice receiving pretreatment (Day 0) versus post-treatment (Day 12) cells from canine case no. 3. Each symbol represents a single animal analyzed at 6 to 8 weeks after transplantation. Primary and secondary transplantations are shown in the left and right graphs, respectively. Mean engraftment is indicated by the horizontal bars. *P = .043; **P = .002. (C) Blood smears for PB samples obtained at the indicated days and cases. (D) Confocal micrograph for canine cells obtained before treatment (day 0) or from day 5 after initial treatment with 50 mg/kg oral dose DMAPT. Top panels show cells stained for NF-κB p65 (yellow) and ToPro3 (blue). Bottom panels show γH2AX (green) and ToPro3 (red).