Figure 1
Cloning efficiency of sorted subpopulations of HCMV-specific CD8+ T cells in limiting dilution analysis. (A) Limiting dilution analysis of sorted CD28−CD45RO− cells, CD28−CD45ROhi cells, and CD28+ CD45ROhi cells (donor 35). The initial number of multimer-positive cells per well was calculated from the initial number of cells per well multiplied by the initial proportion of multimer-positive cells in each subpopulation determined by flow cytometry. (B) Cloning efficiency of each subpopulation in 3 healthy HCMV-seropositive donors; CD28−CD45RO− cells (
), CD28−CD45ROhi cells (
) and CD28+ CD45ROhi cells (
).
