Surface phenotype of M-pDCs compared with FL-pDCs and ex-vivo isolated spleen pDCs. Stained cells from day 6 M-CSF cultures (filled histograms), FL cultures (gray open histograms), or freshly isolated spleen DC (black histograms) were gated on pDCs by selecting for the expression of CD11c and CD45RA or CD45R among the PI-negative cells. The expression of a range of surface markers on the pDC surface is shown. The light gray histograms represent the background staining of the M-pDCs within each stain. All M-pDCs also lacked expression of CD3, CD19, CD49b, and NK1.1. The surface phenotypes shown are from one experiment representative of 2 to 5 experiments for M-pDCs, 2–3 experiments for FL-pDCs, and 2 experiments for spleen pDCs.