T-cell expansion in class A LPD mice. (A) FACS analysis of spleen cells from wild type mice and 2 Stat6VT transgenic mice with class A LPD. Data are shown for staining of 2 TCR Vβ regions on the ordinate. Samples were counterstained for CD3 on the abscissa. Numbers on plots represent percentage of total spleen cells. (B) Total splenocytes from mice of the indicated genotypes were fixed and stained with propidium iodide to analyze DNA content. Numbers shown are the percentages of CD3⁺cells that are in S + G2/M phases of the cell cycle. (C) Isolated splenocytes from wild type or Stat6VT transgenic mice or 2 mice with class A LPD were incubated in 96-well plates and proliferation was assessed using uptake of ³H-thymidine. (D) CD3⁺ splenocytes from mice of the indicated genotypes were tested for propidium iodide exclusion as a measure of apoptosis.