Effect of RTX on PKCζ activity. (A) FL cell lines were treated or not with RTX or IgG at 10 and 50 μg/mL for 24 hours. PKCζ activity was then evaluated by Western blot using anti–phospho-PKCζ antibody. PKCζ was used as control of protein expression. Results are representative of 3 independent experiments. (B) FL cell lines were treated or not with RTX at 50 μg/mL for 24 hours. PKCζ activity was then evaluated by immunokinase assay as described in “Immunokinase assays.” Results are representative of 3 independent experiments.