Effect of PKCζ on mTOR. (A) The effect of PKCζ inhibition on mTOR activity was evaluated by Western blot using anti–phospho-p70S6K (Thr389) antibody in FL cells treated with PS-PKCζ at 40 to 60 μM for 30 minutes. p70S6K was used as control of protein expression. Results are representative of 3 independent experiments. (B) The effect of PKCζ inhibition on mTOR activity was evaluated in Karpas-422 cell lines stably transfected with pcDNA3 (2E10) or DN-PKCζ (5E9, 6E5). p70S6K was used as control of protein expression. Results are representative of 3 independent experiments.