Figure 4
Figure 4. Histologic examination and evaluation of neutrophil infiltration in skin biopsies from anti-Ova injected areas of mice treated with or without FcγR dimers. (Ai) Skin biopsy from anti-Ova antibody–injected area in an untreated mouse with RPA (from Figure 3Ai site 3). The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows the margination of neutrophils along capillary walls (arrows; bottom inset) as well as neutrophilic infiltration into surrounding soft tissue. (Aii) Skin biopsy from PBS-injected area in an untreated mouse with RPA (from Figure 3Ai site 1). No specific pathologic changes are identified. (Aiii) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD16A-Ig before the induction of RPA (from Figure 3Avii site 3). No specific pathologic changes are identified, suggesting abrogation of the Arthus reaction. (Aiv) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD32A-Ig before the induction of RPA (from Figure 3Aii site 3). The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows neutrophilic infiltrates in soft tissue (similar to Figure 3Ai), as well as scattered foci of leukocytoclastic vasculitis (arrows; bottom inset). Top inset: Mast cell degranulation was observed in 10 different sites at 100x magnification and one of the cells is represented in the figure inset. (B) Neutrophils were counted in 10 random sites at 63x magnification and sum of the neutrophils were represented. C: To deplete the complement, mice were twice injected intraperitoneally with cobra venom factor before the initiation of RPA. The mice were injected with CD16A-Ig and CD32A-Ig intravenously (50 μg/mL of blood). After 1 hour, mice were injected intradermally with 25 μg of anti-Ova per site. (Ci) Skin biopsy from anti-Ova antibody injected area in an untreated mouse with RPA. The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows the margination of neutrophils along capillary walls (arrows; bottom inset) as well as neutrophilic infiltration into surrounding soft tissue. (Cii) Skin biopsy from PBS injected area in an untreated mouse with RPA. No specific pathologic changes are identified. (Ciii) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD16A-Ig before the induction of RPA. No specific pathologic changes are identified, suggesting abrogation of the Arthus reaction. (Civ) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD32A-Ig before the induction of RPA. The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows neutrophilic infiltrates in soft tissue, as well as scattered foci of leukocytoclastic vasculitis (arrows; bottom inset). Top inset: Mast cell degranulation was observed in 10 different sites 100x magnification and one of the cells is represented in the Figure inset. (D) Neutrophils were counted in 10 random sites at 63x magnifications and sum of the neutrophils were represented.

Histologic examination and evaluation of neutrophil infiltration in skin biopsies from anti-Ova injected areas of mice treated with or without FcγR dimers. (Ai) Skin biopsy from anti-Ova antibody–injected area in an untreated mouse with RPA (from Figure 3Ai site 3). The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows the margination of neutrophils along capillary walls (arrows; bottom inset) as well as neutrophilic infiltration into surrounding soft tissue. (Aii) Skin biopsy from PBS-injected area in an untreated mouse with RPA (from Figure 3Ai site 1). No specific pathologic changes are identified. (Aiii) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD16A-Ig before the induction of RPA (from Figure 3Avii site 3). No specific pathologic changes are identified, suggesting abrogation of the Arthus reaction. (Aiv) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD32A-Ig before the induction of RPA (from Figure 3Aii site 3). The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows neutrophilic infiltrates in soft tissue (similar to Figure 3Ai), as well as scattered foci of leukocytoclastic vasculitis (arrows; bottom inset). Top inset: Mast cell degranulation was observed in 10 different sites at 100x magnification and one of the cells is represented in the figure inset. (B) Neutrophils were counted in 10 random sites at 63x magnification and sum of the neutrophils were represented. C: To deplete the complement, mice were twice injected intraperitoneally with cobra venom factor before the initiation of RPA. The mice were injected with CD16A-Ig and CD32A-Ig intravenously (50 μg/mL of blood). After 1 hour, mice were injected intradermally with 25 μg of anti-Ova per site. (Ci) Skin biopsy from anti-Ova antibody injected area in an untreated mouse with RPA. The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows the margination of neutrophils along capillary walls (arrows; bottom inset) as well as neutrophilic infiltration into surrounding soft tissue. (Cii) Skin biopsy from PBS injected area in an untreated mouse with RPA. No specific pathologic changes are identified. (Ciii) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD16A-Ig before the induction of RPA. No specific pathologic changes are identified, suggesting abrogation of the Arthus reaction. (Civ) Skin biopsy from anti-Ova antibody injected area in a mouse treated with 50 μg/mL CD32A-Ig before the induction of RPA. The epidermis and dermis are essentially unremarkable. However, the subdermal fat is edematous and shows neutrophilic infiltrates in soft tissue, as well as scattered foci of leukocytoclastic vasculitis (arrows; bottom inset). Top inset: Mast cell degranulation was observed in 10 different sites 100x magnification and one of the cells is represented in the Figure inset. (D) Neutrophils were counted in 10 random sites at 63x magnifications and sum of the neutrophils were represented.

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