Clones PCH101, 206D, and 236A/E7 show similar detection of FOXP3, with 259D being the outlier with low sensitivity. Two separate experiments (panels A-C and panels D-F, respectively) were set up similar to Figure 1. Clones PCH101-PacBlue (A,D), 206D-PacBlue (B) and 236A/E7-PE (E) showed robust FOXP3 detection on activated T cells. In contrast, clone 259D was unable to detect total expression on cells from the same culture at the same time point (C,F). Numbers on plots are percentages of total CD4⁺ T cells.