Surface expression of PSR protein and other adhesion markers on human microendothelial cells: analysis by fluorescence ELISA. (A) Expression of PSR protein on intact HLMECs. Human lung microendothelial cells were subjected to activation for 6 hours with IL-1α (10 ng/mL), TNF-α (10 ng/mL), or LPS (100 ng/mL), or for 24 hours with hypoxia, and then assessed for surface PSR using an ELISA-based assay. Expression profiles of VCAM-1, CD36, and the vitronectin receptor (VnR) in response to endothelial activation are also shown. Values presented are the mean plus or minus SD from 4 to 6 experiments. (B) Expression of PSR protein on intact HRCECs. Human retinal capillary endothelial cells were subjected to activation for 6 hours with IL-1α (10 ng/mL), TNF-α (10 ng/mL), or LPS (100 ng/mL), or for 24 hours with hypoxia, and then assessed for surface PSR using an ELISA-based assay. Expression profiles of VCAM-1, CD36, and the vitronectin receptor (VnR) in response to endothelial activation are also shown. Values presented are the mean plus or minus SD from 3 experiments.