American Society of Hematology

Figure 4

$Figure 4. LSK and myeloid progenitor cell profiles are distorted in Ts65Dn bone marrow. (A) LSK (lineage−, Sca1+, c-kit+) and myeloid progenitor (lineage−, Sca1−, c-kit+) compartments were analyzed in lineage-depleted bone marrow by flow cytometry. Ts65Dn mice displayed an increased percentage of LSK cells. (B) LSK cells stain more brightly for CD34 in Ts65Dn mice. (C) Analysis of myeloid progenitors demonstrates a bias toward the GMP compartment (granulocyte-monocyte progenitor; lineage−, c-kit+, Sca1−, CD34+, FcγR+) and away from the MEP compartment (megakaryocyte-erythrocyte progenitor; lineage−, c-kit+, Sca1−, CD34−, FcγR−). (D) Representative analysis of quiescent LSK cells in Ts65Dn and control bone marrow by pyronin Y/Hoechst staining. Pyronin Y–low cells represent the quiescent LSK fraction. (E) Average values and statistical analysis of quiescent LSK cells in bone marrow. n = 3 Ts65Dn; n = 3 controls. P = .01. Numbers on plots represent the percentage of the total population of cells in the rectangle.$

LSK and myeloid progenitor cell profiles are distorted in Ts65Dn bone marrow. (A) LSK (lineage⁻, Sca1⁺, c-kit⁺) and myeloid progenitor (lineage⁻, Sca1⁻, c-kit⁺) compartments were analyzed in lineage-depleted bone marrow by flow cytometry. Ts65Dn mice displayed an increased percentage of LSK cells. (B) LSK cells stain more brightly for CD34 in Ts65Dn mice. (C) Analysis of myeloid progenitors demonstrates a bias toward the GMP compartment (granulocyte-monocyte progenitor; lineage⁻, c-kit⁺, Sca1⁻, CD34⁺, FcγR⁺) and away from the MEP compartment (megakaryocyte-erythrocyte progenitor; lineage⁻, c-kit⁺, Sca1⁻, CD34⁻, FcγR⁻). (D) Representative analysis of quiescent LSK cells in Ts65Dn and control bone marrow by pyronin Y/Hoechst staining. Pyronin Y–low cells represent the quiescent LSK fraction. (E) Average values and statistical analysis of quiescent LSK cells in bone marrow. n = 3 Ts65Dn; n = 3 controls. P = .01. Numbers on plots represent the percentage of the total population of cells in the rectangle.

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