Altered contribution of distinct hematopoietic cell lineages in BM and spleen in Vav-Cre Rb KO animals. (A) Spleen size in control and Vav-Cre Rb KO animals (n = 7 for control, n = 10 for Vav-Cre Rb KO). (B) Hematoxylin-eosin stain of spleens from control and Vav-Cre Rb KO animals, indicating increased number of neutrophils and erythroblasts in spleen, although the overall architecture of the spleen is still preserved. (C) Flow cytometric analyses of spleens from control and Vav-Cre Rb KO animals for cell-surface markers associated with myeloid cells (Mac-1, Gr-1), B-cells (B220), T and B cells in the spleen (CD5), T cells (CD8a), and erythroid cells (Ter119; n = 5 for both control and Vav-Cre Rb KO). (D) Flow cytometric analyses of BM from control and Vav-Cre Rb KO animals for cell-surface markers associated with myeloid cells (Mac-1, Gr-1), B cells (B220), T cells (CD5, CD8a), and erythroid cells (Ter119; n = 5 for both control and Vav-Cre Rb KO). (E) Cytospins of BM from control and Vav-Cre Rb KO animals, indicating increased frequency of neutrophils in BM of Vav-Cre Rb KO animals. Shown are mean values plus or minus 1 SEM; *P < .05.