Mouse spleen T cells transduced by a DN Rsk2 mutant could not be recovered. (A) Representative flow cytometric profiles of mouse spleen T cells transduced by WT or DN mutants (KK/AA) of mouse Rsk1, Rsk2, and Rsk3. The numbers indicate percentage of the GFP⁺ cells gated on CD3⁺ cells. (B) A summary of 3 independent retroviral transduction experiments. The error bars indicate mean plus or minus standard error of the mean (SEM). The WT and DN mutant for each retroviral construct was labeled as WT and KK/AA, respectively. (C) NIH3T3 cells were infected with 0.2 mL of the same retroviral supernatant produced in 293T⁺ cells as used in panel A and GFP⁺ cells were determined on day 2 after infection. (D) 293T⁺ cells were transfected with pME18S-IL-2Rβ, pME18S-γ_c, pME18S-Jak3, pEIB IL-2Rα PRRIII reporter, pCi NeoStat5a or vector control pCINeo, and pRL-SV together with either WT or the DN mutant (shown as KK/AA) of pCI neo Rsk2, as indicated. IL-2–induced relative luciferase activity from a representative transfection is shown as mean plus or minus SEM from triplicate samples.