Antigen-expressing DC induce expansion of CD8⁺ effector/memory T cells followed by population contraction. (A) CD45.1⁺ OT-I effector/memory cells were transferred i.v. to 11c.OVA (▴) or non-tg (□) recipients, and the total number of OT-I (CD45.1⁺/CD8⁺/Vα2⁺) cells in spleens was determined 3, 7, 14, 28, and 42 days after transfer. Data (mean±SD) are pooled from 2 independent experiments with 2 mice per group at each time point. (B) CD45.1⁺ OT-I effector/memory cells were labeled with CFSE and transferred i.v. to 11c.OVA or non-tg recipients. Three days later, spleen cells were analyzed by flow cytometry. Percentages indicate the proportion of OT-I T cells (CD45.1⁺/CD8⁺) that have divided. Data are representative of 2 mice per group from each of 2 independent experiments. (C) OT-I effector/memory cells (CD45.2⁺) generated from OT-I.bim^−/− (▴,▾) or wild-type (wt) OT-I (■,□) mice were transferred to CD45.1⁺ 11c.OVA (open symbols [▴,□]) or non-tg littermate (closed symbols [▾,■]) recipients. Seven or 28 days after transfer, the total number of OT-I (CD45.2⁺/CD8⁺/Vα2⁺) T cells in spleens was determined. Data (mean±SD) are pooled from 2 independent experiments each with 2 mice per group.