Reduced growth and clonogenicity of MMSET knockdown and knockout MM cells. (A) 105 empty vector control or MMSET shRNA-transduced KMS-11 cells (left) or NCI-H929 cells (right) were plated in triplicate on day 0. Average cell counts (mean ± SD) are presented. (B) MMSET shRNA, but not control shRNA, severely reduces KMS-11 colony formation in methylcellulose. Error bars represent SD. Student t test P values are indicated. (C) Three different MMSET shRNAs reduce methylcellulose colony formation of t(4;14)+ NCI-H929 MM cells. Error bars represent SD. (D) Disruption of the translocated MMSET allele (T-KO) dramatically reduces both the number (top) and size (bottom) of methylcellulose colonies. Arrows indicate T-KO colonies. All photomicrographs are at 4× magnification. Error bars represent SD.
