Figure 5
Figure 5. IL-27 Tg mice exhibit BM dysfunction with enhanced myelopoiesis and impaired development of B-cell lineages. Cells from thymus (A), spleen (B), BM (C), peritoneal cavity (D), and peripheral blood (E) of wild-type and IL-27 Tg no. 1 and/or no. 6 mice (n = 3 each) at 8 weeks of age were stained with various combinations of antibodies and analyzed by flow cytometry. Thymus: DN (CD4−CD8− T), DP (CD4+CD8+ T), CD4SP (CD4+CD8− T), and CD8SP (CD4−CD8+ T). Spleen: CD4+ T, CD8+ T, CD3+ T, pre-B (B220+CD43−IgM−), B (B220+IgM+), myeloid (Mac-1+Gr-1+), and CD41+. BM: pro-B (B220+CD43+), pre-B, immature B (B220lowIgM+), mature B (B220highIgM+), myeloid, and CD41+. Peritoneal cavity: B1a (IgM+CD5+), B1 (IgM+CD23−), and B2 (IgM+CD23+). Data are shown as the mean (± SD). * indicate P < .05, compared with wild-type mice. Numbers represent the percentage of cells in each box or area.

IL-27 Tg mice exhibit BM dysfunction with enhanced myelopoiesis and impaired development of B-cell lineages. Cells from thymus (A), spleen (B), BM (C), peritoneal cavity (D), and peripheral blood (E) of wild-type and IL-27 Tg no. 1 and/or no. 6 mice (n = 3 each) at 8 weeks of age were stained with various combinations of antibodies and analyzed by flow cytometry. Thymus: DN (CD4−CD8− T), DP (CD4+CD8+ T), CD4SP (CD4+CD8− T), and CD8SP (CD4−CD8+ T). Spleen: CD4+ T, CD8+ T, CD3+ T, pre-B (B220+CD43−IgM−), B (B220+IgM+), myeloid (Mac-1+Gr-1+), and CD41+. BM: pro-B (B220+CD43+), pre-B, immature B (B220lowIgM+), mature B (B220highIgM+), myeloid, and CD41+. Peritoneal cavity: B1a (IgM+CD5+), B1 (IgM+CD23−), and B2 (IgM+CD23+). Data are shown as the mean (± SD). * indicate P < .05, compared with wild-type mice. Numbers represent the percentage of cells in each box or area.

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