STAT5 is required for maximal IL-7–stimulated glucose uptake and surface Glut1 levels. IL-7Rα–expressing FL5.12 cells that expressed Bcl-xL to maintain cell viability were transfected with control (shGFP) or STAT5a/b shRNAi and cultured for 30 hours, washed, and cultured in the absence or presence of IL-7 for an additional 14 hours. (A) STAT5, phospho-STAT5 (Y694), and Pim1 were analyzed by immunoblot. (B) IL-7–stimulated glucose uptake (treated − untreated) was measured. (C) FLAG-Glut1 was cotransfected with control or STAT5a/b shRNAi and IL-7–stimulated FLAG-Glut1 level was determined by flow cytometry. Values represent means plus or minus SEM of triplicate samples within the given experiment. By Student t test, *P < .009, **P < .001.