IL-7–mediated survival requires glucose and depends on STAT5 regulation of Akt. (A,B) Bcl-xL (control) and STAT5a-1*6 expression vectors were transfected into (A) FL5.12 cells and (B) FLAG-Glut1–expressing cells. One day after transfection, cells were washed and cultured in the absence of cytokine with DMSO (vehicle) or LY294002 for an additional 14 hours, and (A) glucose uptake and (B) surface FLAG-Glut1 were determined. (C) FL5.12 cells were transfected with control, MyrAkt1, or STAT5a-1*6 expression plasmids, withdrawn from IL-3 in the presence of absence of LY294002, and cell viability was observed over time. (D,E) Nontransgenic or Bcl-xL–transgenic T cells were purified and neglected or cultured in IL-7 in media with 5 mM or 0.05 mM glucose. (D) Bcl-2 protein levels in nontransgenic T cells and (E) cell viability in nontransgenic (wild type) and Bcl-xL–transgenic T cells were observed after one day. Values represent means plus or minus SEM of triplicate samples. By Student t test, *P < .02, **P < .005.