Figure 5
The effect of Akt knockout or Akt inhibition on GPIb-IX-vWF binding–mediated transient platelet adhesion on vWF. Mepacrine-labeled WT or Akt1−/− or Akt2−/− platelets reconstituted in washed red cells in Tyrode solution in the presence of 10 mM EDTA were perfused through a vWF-coated flow chamber at 800 s−1. Transient platelet adhesion to vWF surface were recorded in real time. Number of platelets rolling on vWF (mean ± SD) were counted from 10 randomly selected fields from each of 3 experiments.
