Schematic representation of wild-type human cdb3 and the mutants used in this study. Deleted sequences are represented pictorially as empty boxes. The abbreviation for each construct is listed on the right. For mutants with deleted residues, the parentheses designate the deleted sequence. For mutants with substituted residues, a bold, underlined font indicates the substitutions. Thioredoxin-(1-55) denotes a fusion protein in which thioredoxin is linked to NH₂-terminal residues 1-55 of band 3; kidney (66-379) denotes a construct containing residues 66-379 of band 3; del(1-50) denotes a construct containing residues 51-379 of band 3; del(1-40) denotes a construct containing residues 51-379 of band 3; del(1-31) denotes a construct containing residues 32-379 of band 3; del(1-23) denotes a construct containing residues 24-379 of band 3; del(1-11) denotes a construct containing residues 51-379 of band 3; del(12-23) denotes a construct where NH₂-terminal residues 1-11 are fused to residues 24-379 of band 3; del(29-52) denotes a construct where NH₂-terminal residues 1-28 are fused to residues 53-379 of band 3; 6NNYQN10 denotes a construct containing residues 1-379 of band 3 where residues 6-10 (DDYED) are replaced with NNYQN; 19QQYQN23 and 19QQAQN23 denote NH₂-terminal residues 1-379 where residues 19-23 (EEYED) are replaced with QQYQN or QQAQN; GST-(871-911) denotes a fusion protein where glutathione transferase is linked to the NH₂ terminus of residues 872-911 of band 3 (ie, the polypeptide's extreme COOH terminus).