Bmx regulates TLR-induced TNF and IL-6 production. Macrophages were infected with AdBmx or Ad0 as before. Cells were cultured in complete medium and stimulated with LPS (10 ng/mL), Pam3C-SK4 (100 ng/mL), Malp2 (100 ng/mL), or Flagellin (100 ng/mL) for 18 hours. Cytokine expression in supernatants was assessed by ELISA. (A) LPS-induced TNF, IL-6, IL-8, and IL-10 production was assessed in uninfected, Ad0-infected, and AdBmx-infected macrophages. Values (mean ± SEM) for 4 separate donors normalized to LPS-treated controls are shown. (B) Cytokine production after Pam3C-SK4, Malp2, and Flagellin stimulation was assessed by ELISA. Values (mean ± SEM) for 4 separate donors normalized to Pam3C-SK4- and Malp-2-treated controls are shown. Statistical significance was assessed using Student ttest (*P< .05; **P< .01; ***P< .001).