Effects of TNFα on relative levels of Bfl-1 and Mcl-1. Neutrophils were incubated in the presence or absence of the indicated concentrations of TNFα, or cycloheximide (CHX, 10 μg/mL) for 3 hours. (A) Protein lysates were prepared and Mcl-1 levels were detected by Western blotting. A representative blot of Mcl-1 protein levels from 3 separate experiments is shown. (B) RNA samples were processed and quantitative real-time PCR was performed. Values shown are means (± SD, n = 3)