American Society of Hematology

Figure 5

$Figure 5. Requirement of MITF for mast cell–specific granule formation and the inhibition of the GATA-1:PU.1 interaction in mast cells. (A) Giemsa staining of EYFP+ cells sorted 7 days after the infection of TET-FOG-1 mast cells with LV-CAG-IRES-EYFP (EYFP) and LV-CAG-MITF-IRES-EYFP (MITF). (B) Giemsa staining of LV-CAG-miMITF-IRES-EYFP-infected cells 7 days after infection. The miMITF-infected cells were sorted into 2 fractions, EYFPlow and EYFPhigh (C-E). FACS analysis, Giemsa staining, and RT-PCR analysis of mast cells infected with LV-CAG-IRES-EGFP and LV-CAG-PU.1ΔC-IRES.EGFP. The cells were infected on day 21 and the analysis was carried out on day 28. RT-PCR was performed using the sorted EGFP+ cells. Bars represent 50 μm.$

Requirement of MITF for mast cell–specific granule formation and the inhibition of the GATA-1:PU.1 interaction in mast cells. (A) Giemsa staining of EYFP⁺ cells sorted 7 days after the infection of TET-FOG-1 mast cells with LV-CAG-IRES-EYFP (EYFP) and LV-CAG-MITF-IRES-EYFP (MITF). (B) Giemsa staining of LV-CAG-miMITF-IRES-EYFP-infected cells 7 days after infection. The miMITF-infected cells were sorted into 2 fractions, EYFP^low and EYFP^high (C-E). FACS analysis, Giemsa staining, and RT-PCR analysis of mast cells infected with LV-CAG-IRES-EGFP and LV-CAG-PU.1ΔC-IRES.EGFP. The cells were infected on day 21 and the analysis was carried out on day 28. RT-PCR was performed using the sorted EGFP⁺ cells. Bars represent 50 μm.

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