BH3 profiling determines BCL-2 dependence of ALL cell lines. (A) Summary of binding chart between BH3-only peptides and different antiapoptotic BCL-2 family members, adapted from Certo et al.¹³  (B-E) For BH3 profiling, at least 5 × 10⁸ cells were used to isolate mitochondria by differential centrifugation for each cell line. Mitochondria were then treated with 100 μM BH3 peptides for 35 minutes at room temperature and cytochrome c release assessed by ELISA. Profiles were put in order of most to least primed. (B) RS4;11 cells. (C) SEM-k2 cells. (D) 697 cells. (E) CEM-c1 cells. Values are representative of the mean of 3 independent experiments (+ SD).