Vascular permeability in A2BAR bone marrow chimeric mice.A2BAR bone marrow chimeric mice (A: A2BAR^+/+→A2BAR^+/+ and A2BAR^−/−→A2BAR^−/−; B: A2BAR^−/−→A2BAR^+/+ and A2BAR^+/+→A2BAR^−/−) were administered intravenous Evan blue dye (0.2 mL of 0.5% in PBS) and exposed to room air or normobaric hypoxia (8% O₂, 92% N₂) for 4 hours. Animals were killed and the heart (Ht), colon (Co), kidney (Kd), lung (Lg), spleen (Sp), brain (Br), muscle (Mu) and liver were harvested. Organ Evan blue concentrations were quantified as described in “In vivo hypoxia model.” Data are expressed as means plus or minus SD of Evan blue OD/50 mg wet tissue (n = 6 animals/condition). (A: *P < .05, compared with normoxia; **P < .05, compared with A2BAR^+/+→A2BAR^+/+ bone marrow chimeric mice; and #P < .05, compared with A2BAR^+/+→A2BAR^+/+ bone marrow chimeric mice and normoxia. B: *P < .05, compared with normoxia; **P < .05, compared with A2BAR^−/−→A2BAR^+/+ bone marrow chimeric mice; and #P < .05, compared with A2BAR^−/−→A2BAR^+/+ bone marrow chimeric mice and normoxia). (C) Representative images of abdominal dissections are shown from A2BAR^−/−→A2BAR^+/+ and A2BAR^+/+→A2BAR^−/− bone marrow chimeric mice. Images were obtained using a Canon Power Shot G9 digital camera (Canon).